actin depolymerization factor Search Results


gelsolin / actin-depolymerizing factor(cptc-gelsolin-1)  (Biotium)
N/A
Gelsolin (also known as brevin, Actin-depolymerizing factor or ADF), a protein of leukocytes, platelets and other cells, severs Actin filaments in thepresence of submicromolar calcium, thereby isolating cytoplasmic Actin gels. It is a calcium-regulated, actin-modulating
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anti human gsn  (Proteintech)
93
Proteintech anti human gsn
FIGURE 6 | Silencing of <t>GSN</t> in CAFs inhibits intra-tumoral CD8+ T cells function and drives them towards a dysfunctional state. (A) tSNE plot of iCAF marker gene GSN, and expression of GSN in iCAFs from responders and non-responders. (B) Survival analysis of GSN (in terms of OS) in TCGA-KIRC cohort. (C) Immunohistochemistry (IHC) staining in tumour samples of three non-responders and three responders. (D) Workflow of the co-culture system with primary CAFs (NC, siGSN-1 and siGSN-2), primary ccRCC tumour cells and CD8+ T cells. (E) qRT-PCR analysis of GSN mRNA in three groups (NC, siGSN-1 and siGSN-2). (F) ELISA analysis of the IFN-γ and TNF-α levels in supernatant from the co-culture system. (G) Flow cytometry analysis of IFN-γ, TNF-α, GZMB and Perforin in CD8+ T cells isolated from the co-culture system. (H) GSEA analysis in TCGA-KIRC cohort (low GSN vs. high GSN), as well as in pan-cancer scRNA-seq landscape of ICI therapy (non-responders vs. responders). (I) Representative western blot of GSN, p65, <t>p-p65,</t> <t>IKB,</t> p-IKB and IKKα + β protein expression levels in the co-culture system. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Anti Human Gsn, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+depolymerization+factor/pm40375605-121-32-38?v=Proteintech
Average 93 stars, based on 1 article reviews
anti human gsn - by Bioz Stars, 2026-08
93/100 stars
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anti gelsolin antibody  (Boster Bio)
90
Boster Bio anti gelsolin antibody
FIGURE 6 | Silencing of <t>GSN</t> in CAFs inhibits intra-tumoral CD8+ T cells function and drives them towards a dysfunctional state. (A) tSNE plot of iCAF marker gene GSN, and expression of GSN in iCAFs from responders and non-responders. (B) Survival analysis of GSN (in terms of OS) in TCGA-KIRC cohort. (C) Immunohistochemistry (IHC) staining in tumour samples of three non-responders and three responders. (D) Workflow of the co-culture system with primary CAFs (NC, siGSN-1 and siGSN-2), primary ccRCC tumour cells and CD8+ T cells. (E) qRT-PCR analysis of GSN mRNA in three groups (NC, siGSN-1 and siGSN-2). (F) ELISA analysis of the IFN-γ and TNF-α levels in supernatant from the co-culture system. (G) Flow cytometry analysis of IFN-γ, TNF-α, GZMB and Perforin in CD8+ T cells isolated from the co-culture system. (H) GSEA analysis in TCGA-KIRC cohort (low GSN vs. high GSN), as well as in pan-cancer scRNA-seq landscape of ICI therapy (non-responders vs. responders). (I) Representative western blot of GSN, p65, <t>p-p65,</t> <t>IKB,</t> p-IKB and IKKα + β protein expression levels in the co-culture system. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Anti Gelsolin Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+depolymerization+factor/pmc07609779-167-51-53?v=Boster+Bio
Average 90 stars, based on 1 article reviews
anti gelsolin antibody - by Bioz Stars, 2026-08
90/100 stars
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nicotiana tabacum actin-depolymerizing factor 2  (Maisch GmbH)
90
Maisch GmbH nicotiana tabacum actin-depolymerizing factor 2
FIGURE 6 | Silencing of <t>GSN</t> in CAFs inhibits intra-tumoral CD8+ T cells function and drives them towards a dysfunctional state. (A) tSNE plot of iCAF marker gene GSN, and expression of GSN in iCAFs from responders and non-responders. (B) Survival analysis of GSN (in terms of OS) in TCGA-KIRC cohort. (C) Immunohistochemistry (IHC) staining in tumour samples of three non-responders and three responders. (D) Workflow of the co-culture system with primary CAFs (NC, siGSN-1 and siGSN-2), primary ccRCC tumour cells and CD8+ T cells. (E) qRT-PCR analysis of GSN mRNA in three groups (NC, siGSN-1 and siGSN-2). (F) ELISA analysis of the IFN-γ and TNF-α levels in supernatant from the co-culture system. (G) Flow cytometry analysis of IFN-γ, TNF-α, GZMB and Perforin in CD8+ T cells isolated from the co-culture system. (H) GSEA analysis in TCGA-KIRC cohort (low GSN vs. high GSN), as well as in pan-cancer scRNA-seq landscape of ICI therapy (non-responders vs. responders). (I) Representative western blot of GSN, p65, <t>p-p65,</t> <t>IKB,</t> p-IKB and IKKα + β protein expression levels in the co-culture system. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Nicotiana Tabacum Actin Depolymerizing Factor 2, supplied by Maisch GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+depolymerization+factor/pm28922746-302-11-5?v=Maisch+GmbH
Average 90 stars, based on 1 article reviews
nicotiana tabacum actin-depolymerizing factor 2 - by Bioz Stars, 2026-08
90/100 stars
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actin depolymerizing factor  (Verlag GmbH)
90
Verlag GmbH actin depolymerizing factor
FIGURE 6 | Silencing of <t>GSN</t> in CAFs inhibits intra-tumoral CD8+ T cells function and drives them towards a dysfunctional state. (A) tSNE plot of iCAF marker gene GSN, and expression of GSN in iCAFs from responders and non-responders. (B) Survival analysis of GSN (in terms of OS) in TCGA-KIRC cohort. (C) Immunohistochemistry (IHC) staining in tumour samples of three non-responders and three responders. (D) Workflow of the co-culture system with primary CAFs (NC, siGSN-1 and siGSN-2), primary ccRCC tumour cells and CD8+ T cells. (E) qRT-PCR analysis of GSN mRNA in three groups (NC, siGSN-1 and siGSN-2). (F) ELISA analysis of the IFN-γ and TNF-α levels in supernatant from the co-culture system. (G) Flow cytometry analysis of IFN-γ, TNF-α, GZMB and Perforin in CD8+ T cells isolated from the co-culture system. (H) GSEA analysis in TCGA-KIRC cohort (low GSN vs. high GSN), as well as in pan-cancer scRNA-seq landscape of ICI therapy (non-responders vs. responders). (I) Representative western blot of GSN, p65, <t>p-p65,</t> <t>IKB,</t> p-IKB and IKKα + β protein expression levels in the co-culture system. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Actin Depolymerizing Factor, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+depolymerization+factor/pm19670369-163-3-20?v=Verlag+GmbH
Average 90 stars, based on 1 article reviews
actin depolymerizing factor - by Bioz Stars, 2026-08
90/100 stars
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actin depolymerizing factor (adf)  (Federation of European Neuroscience Societies)
90
Federation of European Neuroscience Societies actin depolymerizing factor (adf)
FIGURE 6 | Silencing of <t>GSN</t> in CAFs inhibits intra-tumoral CD8+ T cells function and drives them towards a dysfunctional state. (A) tSNE plot of iCAF marker gene GSN, and expression of GSN in iCAFs from responders and non-responders. (B) Survival analysis of GSN (in terms of OS) in TCGA-KIRC cohort. (C) Immunohistochemistry (IHC) staining in tumour samples of three non-responders and three responders. (D) Workflow of the co-culture system with primary CAFs (NC, siGSN-1 and siGSN-2), primary ccRCC tumour cells and CD8+ T cells. (E) qRT-PCR analysis of GSN mRNA in three groups (NC, siGSN-1 and siGSN-2). (F) ELISA analysis of the IFN-γ and TNF-α levels in supernatant from the co-culture system. (G) Flow cytometry analysis of IFN-γ, TNF-α, GZMB and Perforin in CD8+ T cells isolated from the co-culture system. (H) GSEA analysis in TCGA-KIRC cohort (low GSN vs. high GSN), as well as in pan-cancer scRNA-seq landscape of ICI therapy (non-responders vs. responders). (I) Representative western blot of GSN, p65, <t>p-p65,</t> <t>IKB,</t> p-IKB and IKKα + β protein expression levels in the co-culture system. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Actin Depolymerizing Factor (Adf), supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+depolymerization+factor/pm10913604-6-9-28?v=Federation+of+European+Neuroscience+Societies
Average 90 stars, based on 1 article reviews
actin depolymerizing factor (adf) - by Bioz Stars, 2026-08
90/100 stars
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actin-depolymerizing factor (adf)-homology domain  (InterPro Inc)
90
InterPro Inc actin-depolymerizing factor (adf)-homology domain
FIGURE 6 | Silencing of <t>GSN</t> in CAFs inhibits intra-tumoral CD8+ T cells function and drives them towards a dysfunctional state. (A) tSNE plot of iCAF marker gene GSN, and expression of GSN in iCAFs from responders and non-responders. (B) Survival analysis of GSN (in terms of OS) in TCGA-KIRC cohort. (C) Immunohistochemistry (IHC) staining in tumour samples of three non-responders and three responders. (D) Workflow of the co-culture system with primary CAFs (NC, siGSN-1 and siGSN-2), primary ccRCC tumour cells and CD8+ T cells. (E) qRT-PCR analysis of GSN mRNA in three groups (NC, siGSN-1 and siGSN-2). (F) ELISA analysis of the IFN-γ and TNF-α levels in supernatant from the co-culture system. (G) Flow cytometry analysis of IFN-γ, TNF-α, GZMB and Perforin in CD8+ T cells isolated from the co-culture system. (H) GSEA analysis in TCGA-KIRC cohort (low GSN vs. high GSN), as well as in pan-cancer scRNA-seq landscape of ICI therapy (non-responders vs. responders). (I) Representative western blot of GSN, p65, <t>p-p65,</t> <t>IKB,</t> p-IKB and IKKα + β protein expression levels in the co-culture system. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Actin Depolymerizing Factor (Adf) Homology Domain, supplied by InterPro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+depolymerization+factor/pm34601007-14-13-18?v=InterPro+Inc
Average 90 stars, based on 1 article reviews
actin-depolymerizing factor (adf)-homology domain - by Bioz Stars, 2026-08
90/100 stars
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recombinant human destrin (actin depolymerizing factor), his-tagged  (Creative BioMart)
N/A
Recombinant human DSTN protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.DSTN is a member of the ADF/Cofilin/destrin superfamily that has the ability to rapidly depolymerize
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destrin actin depolymerizing factor antibody  (Abbexa)
N/A
Destrin Actin Depolymerizing Factor Antibody is a Rabbit Polyclonal antibody against Destrin Actin Depolymerizing Factor
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Image Search Results


FIGURE 6 | Silencing of GSN in CAFs inhibits intra-tumoral CD8+ T cells function and drives them towards a dysfunctional state. (A) tSNE plot of iCAF marker gene GSN, and expression of GSN in iCAFs from responders and non-responders. (B) Survival analysis of GSN (in terms of OS) in TCGA-KIRC cohort. (C) Immunohistochemistry (IHC) staining in tumour samples of three non-responders and three responders. (D) Workflow of the co-culture system with primary CAFs (NC, siGSN-1 and siGSN-2), primary ccRCC tumour cells and CD8+ T cells. (E) qRT-PCR analysis of GSN mRNA in three groups (NC, siGSN-1 and siGSN-2). (F) ELISA analysis of the IFN-γ and TNF-α levels in supernatant from the co-culture system. (G) Flow cytometry analysis of IFN-γ, TNF-α, GZMB and Perforin in CD8+ T cells isolated from the co-culture system. (H) GSEA analysis in TCGA-KIRC cohort (low GSN vs. high GSN), as well as in pan-cancer scRNA-seq landscape of ICI therapy (non-responders vs. responders). (I) Representative western blot of GSN, p65, p-p65, IKB, p-IKB and IKKα + β protein expression levels in the co-culture system. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Journal: Cell proliferation

Article Title: Deciphering the Immunomodulatory Function of GSN + Inflammatory Cancer-Associated Fibroblasts in Renal Cell Carcinoma Immunotherapy: Insights From Pan-Cancer Single-Cell Landscape and Spatial Transcriptomics Analysis.

doi: 10.1111/cpr.70062

Figure Lengend Snippet: FIGURE 6 | Silencing of GSN in CAFs inhibits intra-tumoral CD8+ T cells function and drives them towards a dysfunctional state. (A) tSNE plot of iCAF marker gene GSN, and expression of GSN in iCAFs from responders and non-responders. (B) Survival analysis of GSN (in terms of OS) in TCGA-KIRC cohort. (C) Immunohistochemistry (IHC) staining in tumour samples of three non-responders and three responders. (D) Workflow of the co-culture system with primary CAFs (NC, siGSN-1 and siGSN-2), primary ccRCC tumour cells and CD8+ T cells. (E) qRT-PCR analysis of GSN mRNA in three groups (NC, siGSN-1 and siGSN-2). (F) ELISA analysis of the IFN-γ and TNF-α levels in supernatant from the co-culture system. (G) Flow cytometry analysis of IFN-γ, TNF-α, GZMB and Perforin in CD8+ T cells isolated from the co-culture system. (H) GSEA analysis in TCGA-KIRC cohort (low GSN vs. high GSN), as well as in pan-cancer scRNA-seq landscape of ICI therapy (non-responders vs. responders). (I) Representative western blot of GSN, p65, p-p65, IKB, p-IKB and IKKα + β protein expression levels in the co-culture system. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: These primary antibodies were used in our western blot analysis: p65 (8242, Cell Signalling Technology, CST), p- p65 (3033, CST), IKB (4812, CST), p- IKB (2859, CST), IKKα + β (2697, CST), anti- human GSN (11644- 2- AP, Protein tech), β- tubulin (10094- 1- AP, Protein tech).

Techniques: Marker, Expressing, Immunohistochemistry, Co-Culture Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Isolation, Western Blot